Figure 5.

Expression levels of NF-κB-dependent genes in A549 cells stably expressing SPSB1 upon induction with IL-1β. (A) A549 cells were transduced with lentiviral constructs expressing FLAG-GFP or SPSB1. Cells were harvested in RIPA buffer and subjected to immunoblotting against FLAG and α-tubulin. (B,C) A549 cells stably expressing GFP or SPSB1 were treated with 25 ng/ml of IL-1β for 6 h. The mRNA levels of (B) ICAM-1 and (C) iNOS were measured by qPCR. Data are presented as fold increase over the non-stimulated condition and representative of at least 2 independent experiments, each performed in triplicate. Means and standard deviations are shown and statistical significance was determined using an unpaired Student's t-test (^*p ≤ 0.05).