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IκBα degradation in the presence of SPSB1. A549 cells stably expressing FLAG-GFP or FLAG-SPSB1 were treated with 25 ng/ml of IL-1β for the indicated length of time. Cells were harvested in RIPA buffer supplemented with protease and phosphatase inhibitors and lysates were subjected to immunoblotting against the indicated proteins or the FLAG epitope. Data are representative of 3 experiments performed independently.
