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. 2020 Jan 27;61:2. doi: 10.1186/s40529-019-0278-6

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Symbiotic cultures of D. officinale seeds and seedlings. a Symbiotic seed germination of D. officinale seeds. Protocorms ruptured the seed coats and turned green. Scale bar = 10 mm. b Fungal staining using WGA-FITC (green) showing fungal hyphae (arrowhead) aggregated and penetrating the suspensor end of embryo. Bar = 40 µm. c Longitudinal section of a developing protocorm showing fungal hyphae (arrowhead) colonizing the basal cortical cells of an enlarged embryo. Scale bar = 50 µm. d Longitudinal section of a seedling root showing fungal hyphae (arrowheads) colonizing the epidermis (e) and cortical cells (c). Scale bar = 15 µm