Fig. 3.

MAN promoted apoptosis in A549 cells by regulation of NAMPT/NAD-controlled ROS accumulation. (A) Effect of MAN and co-treatments with NAC/NMN on mitochondrial membrane potentials. (B) NAC and NMN protected cells from apoptosis under MAN treatment indicated by Annexin V-FITC/PI double staining. (C) NAC and NMN restored MAN-induced increases in cleaved caspase expression: (a) results of the immunoblotting assay and (b) quantification of immunoblotting assay. Data are expressed as means ± SD (n = 3). Statistical analyses were performed using the SPSS and differences among different groups were evaluated using ANOVA followed by Tukey post hoc test. *P < 0.05 compared with MAN-treated cells, **P < 0.01 compared with MAN-treated cells