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. 2020 Jan 21;10:3140. doi: 10.3389/fmicb.2019.03140

TABLE 3.

List of the primers used in this study.

Name Sequence (5′-3′) Experiment
pkn rtfw AAAACGATTGGTGCAGCGATC Quantitative RT-PCR analysis
pkn rtrv GCTTATTTTCCGTTCCCGCA
rnpB rtfw TCGTGAGGATAGTGCCACAG
rnpB rtrev GGAAGTTTCTTCCCCAGTCC
hetR dhfw GGATCCCATGAGTAACGACATCGATCTGATC Two-hybrid assays
hetR dhrv GAATTCTTAATCTTCTTTTCTACCAAACACC
pkn dhfw GGATCCCATGAGCCTCTGCATAAACCCTCA
pkn dhrev GAATT CTA CTC TAC ATT GCC GCT ACG CTG
hetR ptac fw GGATCCGAAGGAGATATACCATGAGTAACGACATCGAT pkn22 and hetR co-expression in E. coli
hetR ptac rev AAGCTTTCAGTGGTGGTGGTG
KD pBAD fw GAATTCACCATGTGGAGCCACCCGCAGTTCGAAAAAAGCCTCTGCATAAACCCT
KD pBAD rev AAGCTTCTAGTGCAAATTTTTTTCAAT
pkn mut fw CAAACAGCTAGAGTCCTCAGAGTCCTAATTAACAATCAT pkn22 catalytic residue mutation
pkn mut rev ATGATTGTTAATTAGGACTCTGAGGACTCTAGCTGTTTG
hetR pET28 fw CATATGAGTAACGACATCGATCTG E. coli hetR-his recombinant strains
hetR pET28 rev GGATCCTTAATCTTCTTTTCTACC
hetR S127A fw TCGCGCATTCCCGGTACAGCCCTCACAAGTGAAGAAAAA
hetR S127A rev TTTTTCTTCACTTGTGAGGGCTGTACCGGGAATGCGCGA
hetR S179A fw GAACATCGCATGGAGTTAGCCGAAGCCCTGGCAGAGCAT
hetR S179A rev ATGCTCTGCCAGGGCTTCGGCTAACTCCATGCGATGTTC
hetR pRL fw GGGCCCATGAGTAACGACATCGATCTGATC hetR-phosphoablative substitutions in Nostoc
hetR pRL rev GGATCCTTAATCTTCTTTTCTACCAAACACCATTT
PhetP forward [6FAM]ATTTAGTGGTAAATTCTCTT EMSA assays
PhetP reverse TGAGTTATACGCTATATCAA