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. 2020 Jan 24;3(2):e201900499. doi: 10.26508/lsa.201900499

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© 2020 Schukken et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 2. — (A) Quantitative PCR for Mad2 RNA levels over time in Mad2^cKD RPE1 cells. (B) Western blot for Mad2 levels over time in RPE1 in Mad2^cKD RPE1 cells. (C) Mitotic accumulation of nocodazole-challenged control and Mad2^cKD RPE1 cells measured by phosphorylated histone H3. (D, E) Quantification of mitotic phenotypes of control and Mad2^cKD RPE1 cells assessed by time-lapse imaging for interphase cells (D) and mitotic cells (E). “n” refers to the number of cells analyzed, P-values from chi-squared test. Data also displayed in Fig 5H. (F, G) Single-cell whole-genome sequencing data quantified by AneuFinder for RPE1 control cells (F, 114 cells, 2 aneuploid) and Mad2^cKD RPE1 cells after 5 d of doxycycline treatment (G, 169 cells, 76 aneuploid). Colors refer to the copy number state for each chromosome (fragment).