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. 2020 Jan 21;10:3134. doi: 10.3389/fimmu.2019.03134

Figure 3.

Figure 3

Pulmonary immune cells, cytokines, and total serum IgE in male offspring from vehicle and CORT-treated dams. (A) Schematic representation of dam and offspring treatments. Pregnant females received corticosterone (CORT) or vehicle in drinking water at E12.5 until the end of pregnancy. Male offspring from CORT and vehicle treated dams were treated with saline or HDM consisting of 135 μg of the major allergen Der p 1. (B) Immune cells were quantified, total eosinophils (*p = 0.02, **p < 0.0001), neutrophils (*p < 0.0001), lymphocytes (*p = 0.0009, **p < 0.0001), and alveolar macrophages in BAL of male offspring from vehicle or CORT-treated dams, treated with HDM or saline (n = 6–8). (C) Cytokine and chemokine concentrations in BALF from HDM-treated male offspring from vehicle and CORT-treated dams. Interleukin-4 (IL-4), interleukin-5 (IL-5), interleukin-13 (IL-13), keratinocyte chemoattractant (KC), and monocyte chemoattractant protein-1 (MCP-1) concentrations are reported as pg/mL in BALF. (D) Total IgE in serum of male offspring treated with saline (circle) and HDM (triangle), with concentrations reported as ng/mL (*p < 0.05, n = 3–5).