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. 2020 Jan 21;10:3134. doi: 10.3389/fimmu.2019.03134

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Copyright © 2020 Smith, Paul, McGee, Sinniah, Flom, Jackson-Humbles, Harkema and Racicot.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of pulmonary histopathology in female offspring from vehicle and CORT-treated dams. (A) Representative light photomicrographs of lung tissue sections from male offspring of vehicle and CORT-treated dams stained with hematoxylin and eosin. B, bronchiolar airspace; BV, blood vessel; arrows, immune cell infiltration. (B) Goblet cell hyperplasia was quantified by scoring histological sections based on the percentage of PAS-positive cells lining the bronchoalveolar space: grade 0: <0.5%; grade 1: 0–25%, grade 2: 25–50%, grade 3: 50–75%, grade 4: >75%. B, bronchiolar airspace; BV, blood vessel; arrows, PAS+ cells, scale bar: 100 μm. The mean PAS scores were compared (*p = 0.01, **p = 0.0003, ***p < 0.0001) (n = 5–6).