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. 2020 Jan 21;10:3150. doi: 10.3389/fmicb.2019.03150

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Copyright © 2020 Seyedjavadi, Khani, Eslamifar, Ajdary, Goudarzi, Halabian, Akbari, Zare-Zardini, Imani Fooladi, Amani and Razzaghi-Abyaneh.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of MCh-AMP1 on ROS generation of C. albicans ATCC 10231. Flow cytometric analysis of the intracellular ROS levels was analyzed using DCFH-DA. Cells were treated with different concentrations of MCh-AMP1 (16–64 μg/mL) and amphotericin B (2 μg/mL) for 3 h and stained with DCFH-DA. Histogram analysis shows the percentage of C. albicans cells that produce ROS. The results are based on three independent experiments and represent the average, standard deviation, and P-values (^∗∗p ≤ 0.01, ^∗∗∗p ≤ 0.001) denote statistically significant differences from the effect of MCh-AMP1 on the control cells.