Figure 1.

MRGPRX2-induced Ca²⁺ mobilization is reduced by SOCE inhibition. (A–D) Intracellular Ca²⁺ mobilization in LAD2 human mast cells was determined following incubation with varying concentrations of (A) YM, (B) SKF, (C) Nifedipine, and (D) A425619 for 30 min. Cells were treated with half log doses of the MRGPRX2 agonist cortistatin-14 (CST-14), and changes in fluorescence intensities were recorded for 120 s. Data are plotted as the change in fluorescence [minimum (Min) subtracted from maximum (Max) value] normalized to the maximal change in fluorescence. (E) Traces show SOCE assay following SKF pretreatment. LAD2 cells were suspended in Ca²⁺-free buffer and stimulated with 300 nM CST-14. CaCl₂ (Ca²⁺) at a final concentration of 2 mM was added to the cells at the indicated timepoint. Plotted curves are the average (mean ± S.E.) of 3–6 independent experiments. Data are analyzed with two-way ANOVA. *p < 0.05 and **p < 0.01.