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NF-κB activation in macrophages. Glucosamine (GlcN, 5 mM, 30 min) and thiamet G (ThG, 1 μM 12 h) attenuated LPS-induced NF-κB activation in RAW 264.7 macrophages. NF-κB activity was measured after incubation with LPS (1 μM for 6 h). Data are represented as mean ± SEM of RLU (relative luminescence unit) and are representative of 4–8 experiments. ∗p < 0.05 vs. control; #p < 0.05 vs. LPS. ANOVA followed by Dunnett’s multiple comparisons test.
