Figure 4.

BBI608 inhibits ovarian cancer cell migrative and invasive abilities and pStat3‐dependent apoptosis gene expression. (A) Cell migration capacity of EOC cell SKOV3 was determined by wound healing assay (magnification, ×100). Data are mean ± SD of three independent experiments. (B) and (C) Cell migration and invasion capacity of EOC cell A2780 were determined by transwell assay. The number of migrated and invaded cells was counted, respectively. Data represent means ± SD (n = 3). (D) and (E) Cell migration and invasion capacity of EOC cell SKOV3 were determined by transwell assay. The number of migrated and invaded cells was counted, respectively. Data represent means ± SD (n = 3). (F) and (G) RNA was isolated, reverse transcribed and then analysed by quantitative PCR for mRNA expression of genes related to ovarian cancer cells apoptosis, with GAPDH as control. *P < .05; **P < .01; ***P < .001, ****P < .0001