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A, Western blot analysis of NLRP3 pathway activation after aconitine incubation for 24 h with or without the TNFα inhibitor Enbrel or BNIP3 shRNA. B, Immunofluorescence analysis of BNIP3 and TOM20 to detect mitophagy after aconitine incubation for 24 h with or without the TNFα inhibitor Enbrel, scale bar: 10 μm
