Figure 5.

Bafilomycin attenuates autophagy by inducing mTORC1 activation and does not affect AMPK signalling. Suppression of OPG inhibits OC differentiation in primary OCs and OCLs. (A) TRAP‐positive multinucleated cells were observed after OPG treatment with or without BAF for 3 h (n = 5). Magnification ×200. Scale bar = 20 μm. (B) OC differentiation markers were detected by Western blot after OPG treatment with or without BAF for 3 h and (C) quantitatively analysed. (D) LC3 puncta were observed by confocal microscopy during the OPG treatment with or without BAF for 3 h. Magnification ×630. Scale bar = 20 μm. (E) Autophagy marker proteins, (G) autophagy‐related proteins, (I) mTORC1 and (K) AMPK signalling were detected by Western blot after OPG treatment with or without BAF for 3 h and (F, H, J and L) quantitatively analysed. All data show means ± SD (n = 3). **P < .01 or *P < .05