Figure 1.

CRYAB expression is upregulated during the process of BMSCs osteogenic differentiation. A, A total of 109 differentially expressed genes (52 downregulated genes and 57 upregulated genes) were identified when comparing the differentiation time of 3 or 4 d to that of 1 or 2 h. B&C, Eight downregulated genes and twenty‐five upregulated genes were chosen to further validate the dataset using qRT‐PCR. D‐M, Ten upregulated genes were chosen for functional screen in BMSCs and their expression were downregulated using specific siRNA. N, ALP activity was measured after BMSCs were treated with specific siRNA. O, The expression of CRYAB in BMSCs was measured using Western blotting during the process of osteogenic differentiation