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. 2019 Oct 22;53(1):e12709. doi: 10.1111/cpr.12709

Figure 2.

Figure 2

The role of CRYAB in osteogenic differentiation in vitro. A&B, qRT‐PCR and Western blotting were used to determine the efficacy of CRYAB‐specific shRNA. C, The osteogenic capacity of CRYAB‐downregulated BMSCs was measured by alizarin red staining and ALP staining at day 14 or day 7 after treated with osteogenic induction media. D, The expression of osteogenic markers at mRNA level was measured by qRT‐PCR. E, The expression of osteogenic markers at protein level was measured by Western blotting. F&G, qRT‐PCR and Western blotting were used to determine the efficacy of CRYAB‐specific lentivirus. H, The osteogenic capacity of CRYAB‐upregulated BMSCs was measured by alizarin red staining and ALP staining at day 14 or day 7 after treated with osteogenic induction media. I, The expression of osteogenic markers at mRNA level was measured by qRT‐PCR. J, The expression of osteogenic markers at protein level was measured by Western blotting