Figure 5.

CRYAB is essential for β‐catenin stabilization. A&B, The ubiquitination level of β‐catenin was enhanced by the downregulation of CRYAB and reduced by the upregulation of CRYAB. C, CRYAB could physically interact with β‐catenin in human BMSCs as determined by co‐immunoprecipitation. D&E, The stability of β‐catenin was determined after BMSCs were treated with a protein synthesis inhibitor, cycloheximide (CHX), for indicated hours. F, Representative images of H&E staining of transplants from different groups. The new formed bone was denoted by black arrows. Scale bar represents 100 µm. G, The bone formation was measured qualitatively