Figure 5.

ZEB1‐AS1 promotes the growth and metastasis of COAD by sponging miR‐455‐3p to up‐regulate PAK2 expression. A, The proliferative ability of COAD cells was determined by CCK8 assay. B, The DNA synthesis of COAD cells grown was measured by EdU assay. Scale bar, 100 μm. C, The invasive capacity of COAD cells was assessed by the transwell assay. Scale bar, 50 μm. D, The migratory ability of COAD cells was assessed by the scratch wound assay. Scale bar, 200 μm. E, The excision tumour in nude mice of HT29 xenografts. F, Differences in tumour volume among groups. G, The tumour weight of excised tumour tissues. H, PCR identified miR‐455‐3p and ZEB1‐AS1 expression changes. I, The expression of PAK2 was examined by immunohistochemical staining of sections from the xenograft model. Scale bar, 200 μm.*P < .05, **P < .01, ***P < .001. COAD, colon adenocarcinoma; PAK2, p21‐activated kinases 2; ZEB1‐AS1, zinc finger E‐box‐binding homeobox 1 antisense 1