FIG 2.

Decrease of cccDNA markers in HepAD38 cells by GLP-26. (A) Percent inhibition of HBeAg secretion was visualized by ELISA. Cells with or without drugs were incubated in the absence of tetracycline (Tet) for 7 days, followed by the addition of Tet to the culture of both untreated and drug-treated cells for another 7 days (from day 7 to day 14). Tet ON, cells cultured in the presence of tetracycline only for 14 days. Inhibition (%) of HBeAg secretion was determined relative to untreated Tet off/on control. (B) The levels of HBV DNA and cccDNA were quantified by qPCR and log viral reduction was determined relative to the untreated mock Tet off control. All values represent the average of at least two independent experiments and samples were performed in duplicate ± standard deviation (SD).