Schematic composition and characteristics of ClyJ and its variants. The CHAP EAD is labeled in cyan, the linker is in gray, and the CBM CBD is in purple. The start and end positions of each domain are indicated. The flexibility analysis was performed by using FlexPred online software (https://bio.tools/FlexPred). FEAD, the flexibility of the enzymatically active domain; *FLinker, the flexibility of the linker divided by the number of amino acids in the linker; FCBD, the flexibility of the cell wall binding domain; FTotal, the flexibility of the full-length lysin.