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Thermal unfolding profiles for ClyJ and its variants. The profiles of ClyJ (a), ClyJ-1(b), ClyJ-2 (c), and ClyJ-3 (d) were determined by nanoDSF from 25 to 90°C in the absence or presence of 50 mM choline. Values on the y axis represent the first derivative of the ratio of fluorescence at 350 nm and 330 nm.
