TABLE 3.
Characteristics of strains in the genetic transformation from commensal Neisseria bacteria into N. meningitidis isolates
| Attribute of strain | Strain | Ciprofloxacin MIC (ng/μl) | gyrA allele (mutation) | parC (mutation) | Breakage point positiona
|
Recombinant size (bp) | PubMLST ID | ENA accession no. (rawdata) | |
|---|---|---|---|---|---|---|---|---|---|
| Upstream | Downstream | ||||||||
| Recipient | Nm040 | 0.004 | gyrA23 (none) | SH091 (none) | 58130 | ||||
| Donor 1 | Nei018 | 0.25 | gyrA230 (T91I) | SH015 (none) | −3963 | 1664 | 5,627 | 84198 | ERR3623512 |
| Transformant 1 | Nm040_Nei018_T3 | 0.125 | gyrA230 (T91I) | SH091 (none) | 94049 | ERR3636154 | |||
| Donor 2 | Nei042 | 0.125 | gyrA151 (T91I) | SH003 (none) | −5843 | 2261 | 8,104 | 84206 | ERR3623513 |
| Transformant 2 | Nm040_Nei042_T3 | 0.19 | gyrA151 (T91I) | SH091 (none) | 94050 | ERR3636155 | |||
| Donor 3 | Nei082 | 0.38 | gyrA114 (T91I) | SH001 (none) | −5371 | 666 | 8,037 | 89163 | ERR3623514 |
| Transformant 3 | Nm040_Nei082_T3 | 0.19 | gyrA114 (T91I) | SH091 (none) | 94051 | ERR3636156 | |||
| Donor 4 | Nei110 | 0.38 | gyrA97 (T91I) | SH053 (none) | −1318 | 1988 | 3,306 | 84474 | ERR3623515 |
| Transformant 4 | Nm040_Nei110_T3 | 0.125 | gyrA97 (T91I) | SH091 (none) | 94052 | ERR3636157 | |||
| Donor 5 | Nei139 | 0.5 | gyrA98 (T91I) | SH055 (S87I) | 84477 | ERR3623516 | |||
| Transformant 5.1 | Nm040_Nei139_T3 | 0.19 | gyrA98 (T91I) | SH091 (none) | −6542 | 1646 | 8,188 | 94053 | ERR3636158 |
| Transformant 5.2 | Nm040_Nei139_T7 | 0.5 | gyrA98 (T91I) | SH055 (S87I) | −769 (parC) | 2189 (parC) | 2,808 | 94054 | ERR3636159 |
| Donor 6 | Nei153 | 0.25 | gyrA116 (T91I) | SH001 (none) | −2002 | 2069 | 4,071 | 89164 | ERR3623517 |
| Transformant 6 | Nm040_Nei153_T3 | 0.125 | gyrA116 (T91I) | SH091 (none) | 94055 | ERR3636160 | |||
| Donor 7 | Nei343 | 0.25 | gyrA92 (T91I) | SH010 (none) | −7511 | 656 | 8,167 | 89165 | ERR3623518 |
| Transformant 7 | Nm040_Nei343_T3 | 0.19 | gyrA92 (T91I) | SH091 (none) | 94056 | ERR3636161 | |||
| Donor 8 | Nei437 | 0.25 | gyrA171 (T91I) | SH025 (none) | 136 | 743 | 608 | 84263 | ERR3623519 |
| Transformant 8 | Nm040_Nei437_T1 | 0.125 | gyrA329 (T91I) | SH091 (none) | 94057 | ERR3636162 | |||
a
Positions are given according to the first nucleotide of the gyrA gene of N. meningitidis strain 053442, unless a different gene is indicated in parentheses. A minus sign indicates a position upstream of the gene.