FIG 2.

Expression of KatG^bov in M. marinum (a and b) or M. avium subsp. paratuberculosis (c and d) renders them sensitive to isoniazid (INH). (a) The MIC of wild-type M. marinum-moffet (MMM) to INH (top) is 6.25 μg/ml, whereas that of MMM+katG^bov (mDB85, bottom) is 0.16 μg/ml. (b) mDB85 (MMM+katG^bov, white colonies) and mDB72 (MMM+mCherry, red colonies) were grown on 7H10 plates with INH ranging from 25 to 0.01 μg/ml. (c) Wild-type (K10) M. avium subsp. paratuberculosis is not inhibited by INH at concentrations as high as 25 μg/ml (top), but the MIC for K10+katG^bov (bottom) is between 1.6 and 0.8 μg/ml. (d) Percent inhibition of MAP^wt versus MAP^KatG-bov with increasing concentrations of INH. M. bovis BCG is presented as a control, as well as percent inhibition by the unrelated drug clofazimine.