FIG 4.

Comparison of the bactericidal activity of BDQ and TBAJ-876 against M. tuberculosis. Time-kill curves of 3-, 30-, and 300-fold the MIC₉₀ of TBAJ-876 and BDQ against M. tuberculosis H37Rv are shown. The MIC₉₀ values for BDQ and TBAJ-876 are 400 nM and 125 nM, as described in reference 15. The experiment was carried out three times independently, and the results are presented as mean values with standard deviations. The graph was generated using GraphPad Prism 5 software. The minor differences in CFU of samples at the last time point (21 days), treated with the same fold MIC₉₀ of TBAJ-876 or BDQ, were analyzed for statistical significance (P < 0.05) using unpaired Student's t test. No significant differences were found with the exception of the samples treated with 30-fold MIC₉₀ of the antibiotics.