Skeletal muscle site-specific mitochondrial hydrogen peroxide (mH2O2) emission and susceptibility to opening of the permeability transition pore in control (CON) and type 1 diabetes (T1D) women and men. (A) Complex I-derived mH2O2 (10 mM succinate). (B) Complex I-derived mH2O2 (10 mM pyruvate and 2 mM malate). (C) Complex III-derived mH2O2 (2.5 µM antimycin A). (D) Pyruvate dehydrogenase complex (PDC)-derived mH2O2 (10mM pyruvate and 0.5 µM rotenone). (E) Mitochondrial glycerol-3-phosphate dehydrogenase (mG3PDH)-derived mH2O2 (20mM glycerol-3-phosphate). (F) Mitochondrial Ca2+ retention capacity (mCRC). Right shaded panel in (A) through (F) is the absolute change from each T1D participant relative to the average of their sex-matched control (△T1D-CONavg). Boxes represent the interquartile range, whiskers show the maximum and minimum, the horizontal line indicates the median and the cross indicates the mean. *P < 0.05 versus women, **P < 0.01 versus women. f, female; m, male; Wt, weight. n = 7–8 for CON women; n = 6–7 for CON men; n = 8–10 for T1D women; n = 6–8 for T1D men.