Fig. 2.
Male mice implanted with insulin pellets at 6 wk were tested for glucose tolerance at the indicated number of days after implantation. After an overnight fast and baseline blood glucose measurement, mice were given an injection of glucose solution (2 g/kg ip) and then had blood glucose remeasured at 15, 30, 45, 60, 90, and 120 min after injection. Data are means ± SD; n = 5 mice/group. The slow loss of glycemic control in Akita mice is reflected in the statistical significance by ANOVA testing, in which both baseline fasted blood glucose (time 0) and peak blood glucose values were highly time of implantation dependent (P < 10−8 and P < 10−7, respectively).