Fig. 3.
Akita and control mice at different ages were placed on filter paper for 4 h, filters were photographed under ultraviolet illumination, and images were analyzed for spot numbers and spot volumes by software. A: male Akita and control mice. Data are means ± SD from a minimum of 4 mice/group. Actual numbers of Akita and control mice tested at 1, 2, 4, 7, 12, 15, and 18 mo were 10, 6, 7, 8, 6, 7, and 4 and 5, 6, 5, 5, 4, 4, and 7, respectively. B: female Akita and control mice. Numbers of mice tested at 2, 3, 6, 9, 12, and 18 mo were 13, 9, 6, 8, 8, and 8 and 6, 5, 5, 4, 8, and 5, respectively. Two-way ANOVA with factors “strain” and “age” revealed that male Akita mice were significantly different from controls for all three voiding parameters (P < 10−8), and age was also a highly significant contributor to these variables (P < 0.01 for number of spots and P < 10−8 for the others). Tukey post hoc comparisons showed significance between strains arising at 7, 15, and 7 mo, respectively. Female void parameters were not significantly different at any time point comparison.