Fig. 6.

Metabolic status of diet-induced obese (DIO) mice. Glucose tolerance tests (GTTs) were performed on female (A) and male (B) mice as DIO mice aged. After an overnight fast and baseline blood glucose measurement, mice were given an injection of glucose solution (2 g/kg ip) and then had blood glucose remeasured at 15, 30, 45, 60, 90, and 120 min after injection. Data are means ± SD; n = 5 mice/group. C: insulin tolerance [insuline tolerance test (ITT)] was measured in 4-h fasted female mice at 2 and 16 mo of age. After baseline blood glucose measurement, insulin was injected (0.75 IU/g ip), and glucose was then measured for 75 min. D: male ITT at 16 mo of age. E: blood insulin in female DIO mice was assayed by ELISA after the injection of glucose (2 g/kg ip) at 2 and 16 mo of age. “Strain” differences for time 0 (baseline) measurements were determined by post hoc testing (*P < 0.05).