Fig. 9.
Blood insulin concentrations after a glucose challenge in ob/ob and control mice at 2 and 8 mo of age. Blood insulin was assayed by ELISA after injection of glucose (2 g/kg ip) at 2 and 8 mo of age in both male and female mice. Data are means ± SD; n = 3 mice/group. Two-way ANOVA testing for differences based on “strain” and “age” showed that both male and female baseline insulins were significantly higher in ob/ob mice (P < 0.001 and P < 0.01, respectively). Age was a significant factor for female mice (P < 10−4) but not male mice. **P < 0.01; ***P < 0.001.