Fig. 7.

Daily inhalation of E-cigarette vapor decreases innate immune responses to ip challenge with Pseudomonas aeruginosa (PSA) in mice. A: total leukocytes recruited to the site of bacterial infection in mice exposed to E-cigarette (E-cig) vapor for 1 h daily for 1 mo relative to air controls (n = 5). B: cell differential of leukocytes recovered from the peritoneal space in response to pseudomonal challenge in E-cigarette mice versus controls. Data were analyzed with 2-way analysis of variance with Fisher’s test. C: colony-forming units (CFU) of P. aeruginosa recovered from the ip space at 4 h after infection (n = 6). Data shown are expressed as mean values ± SE. Results were analyzed by Student’s t test. *P < 0.05.