. 2019 Nov 25;318(1):G189–G202. doi: 10.1152/ajpgi.00277.2019

Table 1.

Primers used for real-time PCR of rat amino acid transporter genes

Gene	Protein Alias	Direction	Sequence	Reference No.
GAPDH		Forward	`GGCAAGTTCAACGGCACAGT`	50
GAPDH		Reverse	`TGGTGAAGACGCCAGTAGACTC`	50
SLC1A1	EAAT3	Forward	`TGAGCATCAAGCCTGGTGTCACT`	34
SLC1A1	EAAT3	Reverse	`GGCTTCACTTCTTCACGCTTGGT`	34
SLC1A2	EAAT2	Forward	`TGTCTATGCCGCACACAACT`	21
SLC1A2	EAAT2	Reverse	`TCCTCAACACTGCAGTCAGC`	21
SLC1A3	EAAT1	Forward	`GAGATTGGTAGCGGTGATAATGTG`	60
SLC1A3	EAAT1	Reverse	`CACCCATATCTTCCATCTCAACAA`	60
SLC1A4	ASCT1	Forward	`ACGCGGGACAGATTTTCAC`	58
SLC1A4	ASCT1	Reverse	`ACACCCGCTGCTCCAAC`	58
SLC1A5	ASCT2 or ATB⁰	Forward	`GGAGAAATGGACTGGGTGTG`	59
SLC1A5	ASCT2 or ATB⁰	Reverse	`CCAGCAAGAAGGCTCTGAAT`	59
SLC3A1	rBAT	Forward	`GGGTGTTGATGGGTTTAGTTTC`	61
SLC3A1	rBAT	Reverse	`GTGGTGAAGTCGTGGTACAG`	61
SLC3A2	4F2hc or CD98hc	Forward	`CAGGGTAGGAGCCTCCAACC`	37
SLC3A2	4F2hc or CD98hc	Reverse	`TGCCCTCCTCACGGCTT`	37
SLC6A6	TAUT	Forward	`CGCTCCGCGTGAGAATCAAA`	39
SLC6A6	TAUT	Reverse	`CCGTTCATGAGGGTTGCTCT`	39
SLC6A9	GLYT1	Forward	`CCAGCTTCTCCTTGGTTGTCAT`	16
SLC6A9	GLYT1	Reverse	`AGCATCATCTGAATGTCCTGGAA`	16
SLC6A14	ATB^0,+	Forward	`ATTGGGATAAAGTGACGC`	12
SLC6A14	ATB^0,+	Reverse	`AGAAGAAGGCAAAGTGCTAAG`	12
SLC6A15	B⁰AT2	Forward	`ATAGCCTGTACTTATCTTTGG`	40
SLC6A15	B⁰AT2	Reverse	`AAACCCTCGATCACATCTGG`	40
SLC6A18	XTRP2	Forward	`AGCACGGGGAAGGTGATCTA`	Designed
SLC6A18	XTRP2	Reverse	`GTTAGGCCCTCTGTTGCTCC`	Designed
SLC6A19	B⁰AT1	Forward	`AACCAGAACCAGACAGGCTAT`	38
SLC6A19	B⁰AT1	Reverse	`AGAACACTCCAGGCACAT`	38
SLC6A20	SIT1	Forward	`GTCATCAACAGCTCCACCTC`	52
SLC6A20	SIT1	Reverse	`ATGGCCGCTGTATTTCCAAG`	52
SLC7A1	CAT-1	Forward	`CGTCCCTCCTCATTTGCT`	44
SLC7A1	CAT-1	Reverse	`CTTCCCAGCCCCTGTACT`	44
SLC7A5	LAT1	Forward	`TGGCCGTGAAAGAAACCT`	1
SLC7A5	LAT1	Reverse	`CAGTCCCCAAAGTCAGAAAGA`	1
SLC7A6	y⁺LAT2	Forward	`GGCTGTGACATTTGCTGACC`	Designed
SLC7A6	y⁺LAT2	Reverse	`GACAGAAGGTTCGGGAGGTG`	Designed
SLC7A7	y⁺LAT1	Forward	`CTCGGAACTTGCTTTTGAGG`	22
SLC7A7	y⁺LAT1	Reverse	`CCGAAACAACACGTAGCAAA`	22
SLC7A8	LAT2	Forward	`CTCCACTGGAAAAAAGGTAGCA`	57
SLC7A8	LAT2	Reverse	`TGGTGAATGAAGCCACATCTG`	57
SLC7A9	b^0,+AT	Forward	`TGGGAATATCTCTACTGCGTGTTG`	51
SLC7A9	b^0,+AT	Reverse	`TTACTCTGGGTCCTTCTCTGGTG`	51
SLC7A10	ASC1	Forward	`GTGGGGTCGGCATCATCATT`	Designed
SLC7A10	ASC1	Reverse	`GCGTGTCATGGACTCTGTGAA`	Designed
SLC16A10	TAT1	Forward	`TGCAGCTGTAGGATTCGTTG`	22
SLC16A10	TAT1	Reverse	`GCAGGCAAATACGACTCCAT`	22
SLC36A1	PAT1	Forward	`TGGTTGTACCAGTCGGTGAA`	6
SLC36A1	PAT1	Reverse	`GGCCAGAACACATGTCACAC`	6
SLC36A2	PAT2	Forward	`CACAAGAACGAAACGGTAG`	49
SLC36A2	PAT2	Reverse	`GCCAACAGGGAGAAGATG`	49
SLC38A1	SNAT1	Forward	`TCAGCCTGGTACGTCGATGG`	42
SLC38A1	SNAT1	Reverse	`CCAGGTTCTTCAAGAGACACAG`	42
SLC38A2	SNAT2	Forward	`AGAGCAATTCCAGTATTAGC`	42
SLC38A2	SNAT2	Reverse	`TTAATCTGAGCAATGCGATTGTG`	42
SLC38A3	SNAT3	Forward	`CGGTTCCGATCGTTCTGTTC`	56
SLC38A3	SNAT3	Reverse	`GAACTCCTGGTTCTGAAACAGCAT`	56
SLC38A5	SNAT5	Forward	`GCCCATTTACACGGAACTTTG`	56
SLC38A5	SNAT5	Reverse	`GGACATGTTGGCCACAGCTT`	56
SLC43A1	LAT3	Forward	`CCTGGTAGGCAGTGCCTGCT`	Designed
SLC43A1	LAT3	Reverse	`CCCATTCAAGGACAGTGCCA`	Designed
SLC43A2	LAT4	Forward	`CCTAGGTGCTCACCATCTTCC`	Designed
SLC43A2	LAT4	Reverse	`AAGGACAACCCACACCTCTG`	Designed