Fig. 5.
Conversion of [2H4]CDCA to [2H4]HCA and serum bile acid profiles of chenodeoxycholic acid (CDCA), hyocholic acid (HCA), and hyodeoxycholic acid (HDCA). Term piglets were administered lipid emulsions for 14 days containing standard soy oil (SO) emulsion (IL), α-tocopherol-supplemented SO (VITE), or SO supplemented with daily injections of 10 mg·kg−1·day−1 Rifadin IV (RIF). A: schematic of Cyp3a29 enzymatic conversion of labeled CDCA to HCA. B: calculated fractional synthetic rate of [2H4]HCA from continuous infusion of [2H4]CDCA at a rate of 23 μg·kg−1·day−1 for 12 h. The relative percent serum concentration of CDCA to HCA at day 14 for unconjugated (C), glycine-conjugated (D), and taurine-conjugated (E) bile acids are also shown. Values are expressed as means ± SE; n = 4–7 piglets/group. %FSR significance calculated with repeated measures 2-way ANOVA. *P < 0.05 vs. IL.