Fig. 1.

Neu3^−/− mice are resistant to a decline in body weight after bleomycin treatment. A: tailsnip DNA was prepared from wild-type C57BL/6 (+/+) and Neu3^−/− (−/−) mice, and PCR was used to check for the presence of a 2.1 kBP piece of the Neu3 gene. Molecular mass markers in base pair (BP) are at left. B: PCR was used to check for the presence of a 310 BP fragment of the β-actin gene in the DNA samples. C: percent change in body weight after saline or bleomycin (Bleo) treatment at day 0. Values are means ± SE, n = 6 except for bleomycin-treated C57BL/6, where n = 9. *P < 0.05 comparing bleomycin-treated Neu3^−/− mice to bleomycin-treated C57BL/6 mice (2-way ANOVA, Bonferroni’s test).