Fig. 1.

Experimental design. Mice (8 per group, 4 male, 4 female) were exposed to intranasal (i.n.) dust extract (DE) or saline vehicle on 6 consecutive weekdays and were then euthanized 5 h after the final exposure (A, exposure phase). Mice challenged with DE as above were allowed to recover for an additional 7 days (A, recovery) while being treated with an anti-amphiregulin (AREG) or isotype control antibody (5 treatments ip) during both exposure and recovery phases. Mice were euthanized on day 7 of recovery. In separate experiments, mice (6 per group, male) were challenged with i.n. DE for 8 days, then allowed to recover for 3 days with daily i.n. recombinant AREG treatment, and were euthanized 5 h after the final AREG dose (B).