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. Author manuscript; available in PMC: 2020 Jan 28.
Published in final edited form as: Biochim Biophys Acta Biomembr. 2019 Jan 22;1861(4):819–826. doi: 10.1016/j.bbamem.2019.01.009

Fig. 5.

Fig. 5.

Sterol depletion and cholesterol replacement in HEK 293T cells stably expressing IGF1R (A) or EGFR (B–C). Western blots of untreated cells (ctrl), cells incubated with cholesterol depletion media, or cholesterol depleted cells incubated with MβCD loaded with cholesterol. IR and IGF1R samples were probed for activation-loop phosphorylation (pY1162/pY1163 for IR and pY1135/pY1136 for IGF1R). EGFR samples were probed for kinase domain phosphorylation (pY845) or C-terminal phosphorylation (pY1068). Bands were quantified by densitometry, and the signal for pYpY was divided by the signal for SBP-tag to give intensity values shown below each lane. This experiment was carried out 3 times with similar results.