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. Author manuscript; available in PMC: 2020 Jan 28.
Published in final edited form as: Biochim Biophys Acta Biomembr. 2019 Jan 22;1861(4):819–826. doi: 10.1016/j.bbamem.2019.01.009

Fig. 9.

Fig. 9.

Cholesterol concentration dependence of IR autophosphorylation. Quantification of cholesterol isolated from plasma membranes of untreated (ctrl), cholesterol depleted, or cholesterol substituted cells. Quantification of cholesterol by HP-TLC analysis and quantification of protein levels by Bradford assay. Autophosphorylation activity was normalized to insulin-stimulated untreated (ctrl) cells. The error bars represent standard deviation (n = 4) and the solid line is the best linear fit to the cholesterol standards (r2 = 0.82).