Table 1.
Primers Used for Quantitative Real-Time PCR Analysis
| Gene | Forward Primer | Reverse Primer |
|---|---|---|
| Sirt 1(m) | CGTCTTATCCTCTAGTTCTTGTG | ATCTCCATCAGTCCCAAATCC |
| Acc (m) | GTTCTGTTGGACAACGCCTTCAC | GGAGTCACAGAAGCAGCCCATT |
| Fas (m) | CTGCGATTCTCCTGGCTGTGAA | CAACAACCATAGGCGATTTCTGG |
| Pparα (m) | ACCACTACGGAGTTCACGCATG | GAATCTTGCAGCTCCGATCACAC |
| Acadm (m) | AGGATGACGGAGCAGCCAATGA | GCCGTTGATAACATACTCGTCAC |
| Mttp (m) | CCAGGAAAGGTTCCTCTATGCC | GACTCTCTGATGTCGTTGCTTGC |
| Apob100 (m) | GCATGAGTATGCCAATGGTCTCC | CTGGTTGCCATCTGAAGCCATG |
| Il6 (m) | TACCACTTCACAAGTCGGAGGC | CTGCAAGTGCATCATCGTTGTTC |
| Tnfα (m) | GGTGCCTATGTCTCAGCCTCTT | GCCATAGAACTGATGAGAGGGAG |
| Mcp1 (m) | GCTACAAGAGGATCACCAGCAG | GTCTGGACCCATTCCTTCTTGG |
| Il10 (m) | AGCTCCAAGACCAAGGTGTC | TCCAAGGAGTTGTTTCCGTTA |
| AdipoQ (m) | GATGGCACTCCTGGAGAGAA | TCTCCAGGCTCTCCTTTCCT |
| Pgc1α (m) | GAATCAAGCCACTACAGACACCG | CATCCCTCTTGAGCCTTTCGTG |
| Gapdh (m) | CATCACTGCCACCCAGAAGACTG | ATGCCAGTGAGCTTCCCGTTCAG |
| Acc (h) | TTCACTCCACCTTGTCAGCGGA | GTCAGAGAAGCAGCCCATCACT |
| Fas (h) | GGACCCAGAATACCAAGTGCAG | GTTGCTGGTGAGTGTGCATTCC |
| Srebp1 (h) | ACTTCTGGAGGCATCGCAAGCA | AGGTTCCAGAGGAGGCTACAAG |
| Scd1 (h) | CCTGGTTTCACTTGGAGCTGTG | TGTGGTGAAGTTGATGTGCCAGC |
| Cpt1 (h) | GATCCTGGACAATACCTCGGAG | CTCCACAGCATCAAGAGACTGC |
| Mttp (h) | AGGCTGTCAGAAACTTCCTGGC | GTCTGAGCAGAGGTGACAGCAT |
| Gapdh (h) | GTCTCCTCTGACTTCAACAGCG | ACCACCCTGTTGCTGTAGCCAA |
Notes: Ps: “m” represents mouse; “h” represents human.