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. 2020 Jan 28;21:30. doi: 10.1186/s12931-020-1297-2

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Expression levels of phagocytic receptors on human alveolar macrophages. a FACS gating strategy to delineate alveolar macrophages. Cell suspension was collected by perfusing and lavaging human lung tissues with saline. Alveolar macrophages were detected as live, FSC^hi/SSC^hi, single cell-gated CD45⁺/CD206⁺ cells in the cell suspension. b Representative histograms for the expression levels of FcγRI, CD11b, macrophage scavenger receptor-1 (MSR-1), CD36 and Siglec-1, on FSC^hi/SSC^hi/CD45⁺/CD206⁺ alveolar macrophages of control never-smokers (CNS, n = 11), control ex-smokers (CES, n = 9) and COPD ex-smokers (COPD, n = 11). Specific staining, open; isotype control, shaded