Fig. 5.

Cytotoxicity of rBacCPA250–363H6 in CaCo-2 cells. Cells were treated as described in the methods section with rBacCPa250–363H6, PLC and culture supernatant of C. perfringens C-5560/18 strain. a The viability of cells, as determined by the MTS assay, after treating cells with indicated concentrations of rBacCPA250–363H6 and PLC toxins and (b) with a serially diluted solution of the supernatant of C. perfringens C-5560/18 strain. The data represent the mean of three independent experiments