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. 2020 Jan 28;20:7. doi: 10.1186/s12896-019-0597-4

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Characterization of polyclonal and monoclonal antibodies against rBacCPA250–363H6 by western blotting. The MAbs (section a-d) and PAbs (section e) generated were tested against culture supernatant of the C. perfringens CPA toxin producer. The name of the tested antibody is shown below the corresponding picture. The MW marker is shown on the left. Section a and b: Culture supernatant of C. pefringens strain C-5560/18 (lane 1), culture supernatant of C. septicum, used as a negative control (lane 2), and a mixture of full length PLC and rBacCPA250–363H6 (lane 3). Section c and d: Culture supernatant of C. perfringens strain C-5560/18 (lane 1), culture supernatant of C. septicum, used as a negative control (lane 2), full length PLC (lane 3), and rBacCPA250–363H6 (lane 4). Section e: Rabbit anti-rBacCPA250–363H6 PAbs and anti-PLC (Star Fish): mixture of rBacCPA250–363H6 and PLC (lane 1), different culture supernatants of C. perfringens CPA protein producers (lane 3–5), and culture supernatant of C. septicum, used as a negative control (lane 2). Section f: MAb 318F11B7 tested against recombinant and native CPA: mixture of rBacCPA250–363H6 and PLC (lane 1), different culture supernatants of C. perfringens CPA protein producers (lane 2–4), and culture supernatant of C. septicum, used as a negative control (lane 5)