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. 2020 Jan 27;18:10. doi: 10.1186/s12915-020-0740-7

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — The TRAP1 complex is induced by OXPHOS inhibition. a Immunoblot of a native gel analysis of TRAP1 complexes from HEK293T cells grown with different carbon sources under normoxia or hypoxia (1% O₂) overnight. Lower panel: graphical representation of the levels of the TRAP1 complex shown in the upper panels; band intensities were quantitated using ImageJ. b Immunoblot of a native gel analysis of TRAP1 complexes from cells subjected to hypoxia (1% O₂) or an oligomycin (10 μM) treatment in parallel (in normoxia) for 2, 4, 6, and 8 h. The lower panel shows the quantitation. All quantitations with ImageJ shown are for a single native gel; similar results were obtained in three independent experiments