TABLE 1.
Specific primers for genes encoding antifungal peptides.
| Peptide | Gene | Primer name | Primers (5′–3′) | Tm (°C) | bp |
| Surfactin | srfAA | srfAA-F | TCGGGACAGGAAGACATCAT | 60 | 201 |
| srfAA-R | CCACTCAAACGGATAATCCTGA | ||||
| Fengycin | fenB | fenB-F | CTATAGTTTGTTGACGGCTC | 55 | 1400 |
| fenB-R | CAGCACTGGTTCTTGTCGCA | ||||
| fenD | fenD-F | GGCCCGTTCTCTAAATCCAT | 60 | 269 | |
| fenD-R | GTCATGCTGACGAGAGCAAA | ||||
| Iturin | ITUDI | ITUDI-F | GATGCGATCTCCTTGGATGT | 59 | 650 |
| ITUDI-R | ATCGTCATGTGCTGCTTGAG | ||||
| ituD | ituD-F | TTGAAYGTCAGYGCSCCTTT | 58 | 482 | |
| ituD-R | TGCGMAAATAATGGSGTCGT | ||||
| ituA | ituA-F | ATGAAAATTTACGGAGTATATATG | 53 | 1150 | |
| ituA-R | TTATAACAGCTCTTCATACGTT | ||||
| ituC | ituC-F | GGCTGCTGCAGATGCTTTAT | 60 | 423 | |
| ituC-R | TCGCAGATAATCGCAGTGAG | ||||
| Mycosubtilin | mycB | mycB-F | ATGTCGGTGTTTAAAAATCAAGTAACG | 60 | 2024 |
| mycB-R | TTAGGACGCCAGCAGTTCTTCTATTGA | ||||
| Bacillomycin | bmyB | bmyB-F | GAATCCCGTTGTTCTCCAAA | 60 | 370 |
| bmyB-R | GCGGGTATTGAATGCTTGTT | ||||
| bmyD | bmyD-F | TTGAAYGTCAGYGCSCCTTT | 51 | 482 | |
| bmyD-R | TGCGMAAATAATGGSGTCGT | ||||
| Bacilysin | BACD | BACD-F | AAAAACAGTATTGGTYATCGCTGA | 52 | 749 |
| BACD-R | CCATGATGCCTTCKATRCTGAT | ||||
| BACAB | BACAB-F | CTTCTCCAAGGGGTGAACAG | 61 | 815 | |
| BACAB-R | TGTAGGTTTCACCGGCTTTC | ||||
| BAC | BAC-F | CAGCTCATGGGAATGCTTTT | 60 | 498 | |
| BAC-R | CTCGGTCCTGAAGGGACAAG | ||||
| Flagellin | hag | hag-F | ATGAGAATCAACCACAATATCGC | 54 | 1210 |
| hag-R | TTAACCTTTAAGCAATTGAAGAA | ||||
| Antimicrobial component | tasA | tasA-F | ATGGGTATGAAAAAGAAATTAAG | 52 | 786 |
| tasA-R | TTAGTTTTTATCCTCACTGTGA |