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. 2019 Nov 21;173(2):313–335. doi: 10.1093/toxsci/kfz233

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Figure 10. — Quantitative comparison of BrdU incorporation in the testicular cell co-culture treated with BPA, BPS, BPAF, and TBBPA. Co-cultures were treated with various concentrations of BPA and BPS (5, 10, 25, 50, and 100 μM) and BPAF and TBBPA (1, 2.5, 5, 10, and 15 μM) for 24, 48, and 72 h. Cells treated with vehicle (0.05% DMSO) were used as controls (0 μM). Representative scatterplot of nuclear area (µm²) and total intensity of BrdU were shown in (A). BrdU-positive cells were identified as having a total intensity of BrdU over 20 000 pixels. Absolute BrdU-positive cell number and percentage of BrdU-positive cells were shown in (B). Geometric mean (GMean) and 90% quantiles of total intensity were calculated (C). Data were presented as mean ± SD, n = 8. Four replicates in 2 independent experiments were included. Statistical analysis was conducted by 2-way ANOVA followed by Tukey-Kramer multiple comparisons (*p < .05, **p < .01). Abbreviations: BPA, bisphenol A; BPAF, bisphenol AF; BPS, bisphenol S; TBBPA, tetrabromobisphenol A.

Figure 10. — Quantitative comparison of BrdU incorporation in the testicular cell co-culture treated with BPA, BPS, BPAF, and TBBPA. Co-cultures were treated with various concentrations of BPA and BPS (5, 10, 25, 50, and 100 μM) and BPAF and TBBPA (1, 2.5, 5, 10, and 15 μM) for 24, 48, and 72 h. Cells treated with vehicle (0.05% DMSO) were used as controls (0 μM). Representative scatterplot of nuclear area (µm²) and total intensity of BrdU were shown in (A). BrdU-positive cells were identified as having a total intensity of BrdU over 20 000 pixels. Absolute BrdU-positive cell number and percentage of BrdU-positive cells were shown in (B). Geometric mean (GMean) and 90% quantiles of total intensity were calculated (C). Data were presented as mean ± SD, n = 8. Four replicates in 2 independent experiments were included. Statistical analysis was conducted by 2-way ANOVA followed by Tukey-Kramer multiple comparisons (*p < .05, **p < .01). Abbreviations: BPA, bisphenol A; BPAF, bisphenol AF; BPS, bisphenol S; TBBPA, tetrabromobisphenol A.