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. 2019 Nov 21;173(2):313–335. doi: 10.1093/toxsci/kfz233

Figure 2.

Figure 2.

Cell viability determined by NR uptake assay (A–C) and cell number determined by the HCA (D–F) in the testicular cell co-cultures treated with BPA, BPS, BPAF, and TBBPA. Testicular cells co-cultures were treated with various concentrations of BPA and BPS (25, 50, 100, 200, and 400 μM), and BPAF and TBBPA (2.5, 5, 10, 25, and 50 μM) for 24 (A, D), 48 (B, E), and 72 h (C, F). Cells treated with the vehicle (0.05% DMSO) were used as vehicle controls (0 μM). Neutral red assay and HCA-based quantification of cell viability and cell number were described in Materials and Methods. Data were expressed as mean ± SD, n = 8. Four replicates in 2 independent experiments were included. Statistical analysis was conducted by 2-way ANOVA followed by Tukey-Kramer multiple comparisons (*p < .05, ** p < .01). Abbreviations: BPA, bisphenol A; BPAF, bisphenol AF; BPS, bisphenol S; TBBPA, tetrabromobisphenol A; HCA, high-content analysis.