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The effect of fasudil pretreatment on BMSCs. To investigate the effect of fasudil (15μg/mL) on BMSCs, the cells were cultured with or without fasudil for 72 h. (A), EdU staining determined the effect of fasudil on the proliferation of BMSCs. Scale bar: 50μm. (B), Quantification analysis of EdU staining. C, Extracellular release of BDNF was measured using ELISA. Results are shown as mean ± SEM, and multiple comparisons were analyzed by one-way ANOVA. *P < 0.05.
