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. 2020 Jan 23;16:249–262. doi: 10.2147/NDT.S238646

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© 2020 Tang et al.

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Expression of iNOS and Arg-1 in the substantia nigra (SN) were determined by immunostaining and Western blot. All slices were independently and blindly examined under a fluorescence light microscope by two investigators. (A) Immunofluorescence of iNOS staining in SN (Red=iNOS; Blue=DAPI). Scale bar: 20μm. (B) Immunofluorescence of Arg-1 staining in SN (Red=iNOS; Blue=DAPI). Scale bar: 20μm. (C) iNOS protein expression in brain by Western blot. (D) Arg-1 protein expression in brain by Western blot. (E) The ratio of iNOS/Arg-1 in brain. Semi-quantification of relative iNOS and Arg-1 protein was obtained from among at least six different animals. *P < 0.05.