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. 2020 Jan 23;16:249–262. doi: 10.2147/NDT.S238646

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© 2020 Tang et al.

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Expression of Nestin+ cells in the subventricular zones (SVZ) after intranasal application of BMSCs was determined by immunostaining. All slices were independently and blindly examined under a fluorescence light microscope by two investigators. (A) Immunofluorescence of Nestin+ cells in the SVZ. Scale bar: 50μm. (B) Numbers of Nestin+ cells in the SVZ. Results are shown as mean ± SEM of 6 mice each group, and multiple comparisons were analyzed by one-way ANOVA. **P < 0.01.