Table 1.
Primers and PCR Products of PZA-Associated Genes with Brief Explanatory Notes
| Gene | Protein | Functional Activity | Primer Name | Oligonucleotide Sequence (5ʹ→3ʹ) | Product Size ~ (−200 to + 200) |
|---|---|---|---|---|---|
| pncA (Rv2043c) | Pyrazinamidase/nicotinamidase (PZase) | Convert amide into acid (PZA into POA) | pncA FpncA R |
TCGCTCACTACATCACCGGC TCGTAGAAGCGGCCGATGGC |
892 bp |
| rpsA (Rv1630) | 30S ribosomal protein S1 (RpsA) |
Trans-translation | rpsA F rpsA R | ACTGAGTGCCGAGCGTGCATC ACCGAACGCGTCGACCAGCG | 1800 bp |
| panD (Rv3601c) | Aspartate alpha-decarboxylase (PanD) | Pantothenate biosynthesis | panD F panD R | TCGACTACCTGGAGCTGCGC TCGATCGTCAGTGCCAGTTC | 755 bp |
| Rv2783c | Bifunctional protein polyribonucleotide Nucleotidyltransferase (GpsI, Pnpase) and synthesize and hydrolyze (p)ppGpp |
Synthesis/degradation of ssDNA/ssRNA and (p)ppGpp | gpsI F gpsI R |
ATTCAGACCTTTTCTCCTGGG GTCGACTTGAACAGCAAATG |
2547 bp |
| clpC1 (Rv3596c) | ATP-dependent protease ATP-binding subunit (ClpC1) |
Hydrolyses proteins in presence of ATP |
clpC1 F clpC1 R | ACGCTTGGGTGGTTTTCTCGTT ACAAACCGACGTCAGCAGAGT | 2816 bp |
| *Rv2044c-pncA-Rv2042c | Conserved hypothetical protein- PZase- Conserved protein | EO pncA F EO pncA R | GTGCCGCATCGAGTTCGATCCGCA GATATCGGGATAGCGCCGCTGGA | 2070 bp | |
Notes: Primers were based on the M. tuberculosis H37Rv genome sequence (Accession No.: NC_000962; Version: NC_000962.3). *Entire operon of pncA.