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. 2020 Jan 24;13:757–767. doi: 10.2147/OTT.S224550

Figure 3.

Figure 3

The target association between SNHG1 and miR-204. (A) starBase database predicted the binding sites of SNHG1 and miR-204. (B and C) Luciferase activity was determined in EC9706 and KYSE150 cells co-transfected with SNHG1-WT or SNHG1-MUT and miR-204 or miR-NC. (D and E) qRT-PCR assay was performed to detect SNHG1 abundance in EC9706 and KYSE150 cells transfected with miR-204 or miR-NC after RIP assay. (F) The expression of miR-204 was detected in esophageal squamous cell cancer cells compared with normal esophageal epithelium cell. (G and H) The level of miR-204 was measured in EC9706 and KYSE150 cells transfected with pcDNA, SNHG1, si-NC or si-SNHG1. *P<0.05, **P<0.01, ***P<0.001.