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. 2020 Jan 24;13:757–767. doi: 10.2147/OTT.S224550

Figure 5.

Figure 5

The target association between miR-204 and HOXC8. (A) The seed sites of miR-204 and HOXC8 were predicted by starBase database. Luciferase reporter assay (B and C) and RIP assay (D and E) were conducted to confirm the bind of miR-204 and HOXC8 in EC9706 and KYSE150 cells. (F) The expression of HOXC8 mRNA was measured in esophageal squamous cell cancer cells compared with normal esophageal epithelium cell. (G and H) The protein level of HOXC8 was examined in EC9706 and KYSE150 cells transfected with miR-NC, miR-204, anti-miR-NC or anti-miR-204. *P<0.05, **P<0.01, ***P<0.001.